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conical bottom 384 well plate  (Greiner Bio)


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    Structured Review

    Greiner Bio conical bottom 384 well plate
    Conical Bottom 384 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/conical+bottom+plate/Microplate+384+Deep+Well+Pp/pmc12483515-310-29-32
    Average 93 stars, based on 14 article reviews
    conical bottom 384 well plate - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.
    Article Snippet: After 45 min incubation at room temperature, plates were washed three times in PBS 0.05% and twice with PBS before adding alkaline phosphatase substrate (170-6432; Biorad). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865- 18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865- 18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA).

    Article Title: Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism.
    Article Snippet: .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Retrograde And Anterograde Transport Of LAT-Vesicles During The Immunological Synapse Formation: Defining The Finely-Tuned Mechanism
    Article Snippet: .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Tethering of vesicles to the Golgi by GMAP210 controls LAT delivery to the immune synapse
    Article Snippet: Supernatants were recovered after 6 h and tested for IFN-γ by ELISA (BD OptEIA, 555142). .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism
    Article Snippet: .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation
    Article Snippet: After 45 min incubation at room temperature, plates were washed three times in PBS 0.05% and twice with PBS before adding alkaline phosphatase substrate (170-6432; Biorad). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865-18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865-18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA).

    FACS:

    Article Title: Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.
    Article Snippet: After 45 min incubation at room temperature, plates were washed three times in PBS 0.05% and twice with PBS before adding alkaline phosphatase substrate (170-6432; Biorad). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865- 18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865- 18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA).

    Article Title: Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism.
    Article Snippet: .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Retrograde And Anterograde Transport Of LAT-Vesicles During The Immunological Synapse Formation: Defining The Finely-Tuned Mechanism
    Article Snippet: .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Tethering of vesicles to the Golgi by GMAP210 controls LAT delivery to the immune synapse
    Article Snippet: Supernatants were recovered after 6 h and tested for IFN-γ by ELISA (BD OptEIA, 555142). .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism
    Article Snippet: .. Cells were centrifuged and transferred to conical bottom plate (Greiner Bio-One, 650101), stained for 20 min in cold PBS with Fixable Violet Dead Cell Stain Kit (1/4000, Invitrogen, L34955) and washed in FACS Buffer (PBS 0.5% BSA 2 mM EDTA). ..

    Article Title: Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation
    Article Snippet: After 45 min incubation at room temperature, plates were washed three times in PBS 0.05% and twice with PBS before adding alkaline phosphatase substrate (170-6432; Biorad). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865-18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA). .. In all conditions, cells were centrifuged and transferred to a conical-bottom plate (650101; Greiner Bio-One), stained in PBS with Fixable Viability Dye eFluor 780 (1:4,000, 65-0865-18; eBioscience), and washed in FACS Buffer (1× PBS, 0.5% BSA, 2 mM EDTA).

    other:

    Article Title: Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) 4: DNA Biotinylation, Precipitation, and Arraying of Samples.
    Article Snippet: Cold Spring Harb Protoc Andrew J.. Link and Joshua LaBaer 4: DNA Biotinylation, Precipitation, and Arraying of Samples Construction of Nucleic Acid Programmable Protein Arrays (NAPPA) Service Email Alerting click here.. Receive free email alerts when new articles cite this article Categories Subject Cold Spring Harbor Protocols.



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